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DNA from matching normal lymphocytes for samples from Southampton were extracted as described previously [33].
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Library preparation was performed as previously described [ 9] following the Illumina genomic DNA library preparation protocol (Illumina, San Diego, CA) using an input of 200 ng of tumour or matched normal lymphocyte DNA.
Moreover, matching normal DNA is not mandatory in most cases.
Only GSE21124 data set was analyzed by matching normal samples.
SNP array data from matched blood lymphocytes or matched normal tissue were also available for 494 patients.
Fifty frozen samples of primary endometrial carcinomas and those of their matched normal tissues (including cervix and/or lymphocytes) obtained after surgery were retrieved without specific selection from our tissue bank and used for DNA analysis in this study (approved (No.: EC 1517-00) by the Ethics Committee of the University of Hong Kong).
As germline control, matched normal DNA was used where available (Patient 9) or a normal DNA pool was created from normal lymphocyte DNA extracted from 44 healthy individuals.
All the cases had matched normal tissues.
All matched normal DNA showed KRAS and BRAF wild-type.
Variant calling is commonly performed against a matched normal.
All matched normal DNA showed KRAS wild-type.
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