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Specifically, in order to find out which region of the query matches which region(s) of the hit sequence, the matching coordinates of each HSP must be mapped on both the query and hit sequences.
Note that there is a small overlap between the matching coordinates along the reference sequence (5 nucleotide between the first two matches, and 3 nucleotides between the last two), which is an ostensible deviation from strict colinearity between the three hits on the maize chromosome and the reference sequence.
Such a mapping process can be tedious if done by hand (e.g., manually extracting each set of matching coordinates and drawing them on pieces of paper).
Definitions (X A, Y A ) and (X B, Y B ) present the matching coordinates of source A and B and compute the slope S: S = Y B − Y A X B − X A = ΔY ΔX (2) II.
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From all matched coordinates in S x, we now choose the ordered subset ( {check{S}}_x=left right.{left({S}_xright)}_i{left)right.}_{i=1}^{N_x} ) consisting of the ( {N}_x:= min left{ left({S}_xright),{N}_{3mathrm{D}}right} ) most similar blocks.
31 We performed genomic liftover, using the R package rtracklayer for conversion to the assembly NCBI 36 coordinates, to match the coordinates of the CNV dataset.
With the old transmitters, including the one on the single-engine Super Decathlon flown by Mr. Fossett, Coast Guard and Civil Air Patrol officers must visit the site matching the coordinates of each distress signal to discover if a real problem exists.
We considered only splice sites supported by matching intron coordinates of spliced transcript alignment evidence.
The 2D candidate locations from each view were combined into 3D candidates by matching their coordinates on the shared axis (horizontal axis).
We identified the SNPs in the CpG islands by matching the coordinates of the SNPs with those of the CpG islands in the reference sequences.
Not matching on coordinates avoids the problem of having the system and the gold standard denoting two different toponyms but referring to the same coordinates; for instance, a city and its state may have the same geo-coordinates in GeoNames but they refer to different locations and hence will have different place IDs.
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