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Resting cells treated with matching concentration of dsDNA prepared by annealing free plus or minus oligo with the corresponding fluorescence coupled complementary oligo were used as a control.
Quantification of the saponin is expressed in μg/g of LJ and determined by a standard curve from a plot of the peak area and matching concentration of the standard solution.
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In 65 female White Zealand rabbits, the intramedullary cavity was inoculated with matching concentrations of Staphylococcus aureus, and one of the three nails was inserted.
When cells were treated with these reagents, matching concentrations of vehicle were used as the control and the final concentration of DMSO was kept at or below 0.1% in all experiments.
For iHIV activation, MDDCs were stimulated for 24 h with AT-2 iHIV particles (50 µg/ml p24) or matching concentrations of control microvesicles (gift from J. Lifson) and then pulsed for 1 h with CMV peptide pools.
In brief, after cells were seeded in 96-well plates at 3000 cells/well in their culture media overnight, the cells were treated in various concentrations of glutamine (0, 0.5, 2.0, and 5.0 mM) for 24 h and then the cells were cultured with 2-NBDG (100 μg/ml) in glucose-free media with matching concentrations of glutamine for 40 min.
The control received a matched concentration of normal mouse immunoglobulin diluted in PBS/T 1% BSA.
siRNA treatments were carried out in 6-well plates, each well seeded with 1×10 hTERT-1604 cells or 1×10 NIH 3T3 cells, before reverse transfection using Dharmafect 1 (Thermo Fisher Scientific) and 80 nM (NIH 3T3) or 100 nM hTERT-16044) ON-TARGETplus SMARTpool DNMT1 siRNA or a matched concentration of scrambled control (Thermo Fisher Scientific).
The biological activity in the samples was compared with control samples that were freshly spiked with matched concentrations of catumaxomab.
Values plotted were normalized to vehicle-treated cells transfected with a scrambled siRNA of matching concentration.
The simulation results are analyzed from three perspectives: phonon match, concentration of N doping, and distribution of N doping.
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