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Moreover, in S. vulgaris, the part matching ATMG00860 is contained in a match with the transposon sequence Q3I6J4_SILLA, which also includes the sequence matching accession number AT3G01410 (Table 1).
In the two other cases (FSHB, FSHBMS, and CSPS101 matching accession Genbank M83753; NCandMB085d matchingtchinGenbanknk accession X16451), markers in the set were separated by several markers after initial map construction.
Moreover, with every new sample passing through the analysis, the database of reference SSR profiles is enlarged and the probability of identifying the closest relative or exactly matching accession is enhanced.
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Finally, I cross-matched accessions between the SILVA, greengenes, and RDP databases to obtain a collection of 200,433 sequences.
Besides, there were also a quarter of annotated all-unigenes with matched accessions from its legume relatives (Table 2).
Using a list built with the compartmentization algorithm resulted in 8923 (95%) matching accessions.
In two instances (MB064 and HBBMS matching Genbank accession AC130787; T608B5 and SP608B5 matching Genbank accession NM_001752), markers in the set were placed adjacent to each other by the map building routine.
A basic keyword-based search functionality (allowing wildcards) is available for retrieving protein sequences with matching UniProtKB Accession(s)/Entry Name(s) or gene name(s).
Each protein was also matched to its GeneID at NCBI [ 42], if available, by accessing the European Bioinformatics Institute [ 43], downloading the ipi.HUMAN.xrefs.gz IPI dataset [ 44], and matching each accession number to its corresponding gene ID number.
For the remaining samples DNA was successfully extracted and amplified, and all 15 were successfully sequenced as E. granulosus G1 (≥99% match, accession number DQ408422.1).
From the remaining samples, DNA was successfully extracted, analysed by PCR and sequenced, and all 17 were identified as T. hydatigena (≥99% match, accession number GQ228819.1).
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