Sentence examples for matching a transcript from inspiring English sources

To ensure unequivocal probe-to-gene assignment, we re-annotated the HG-U133 plus 2.0 probe sets with custom chip definition files (CDF) [18], [19], where each of the 18,900 genes in the microarray is associated with a single, custom probeset that includes only probes unequivocally matching a transcript.

In the first step individual probe sequences are aligned to the corresponding genome sequence and in the second step probesets having at least half of their probes matching a transcript are annotated with the associated transcript.

Fewer assembled G. nigrifrons transcripts were identified in this study than in the initial 2012 assembly of Chen et al. (37,548 vs. 38,240), with <1% (n = 246) of the transcripts from Chen et al. not matching a transcript in the current assembly (BLASTn, E-value <10-10 <10-10

Overall, although the normalization procedure was successful, as judged from the gel images before and after normalization, the dynamic range of library, expressed as the total number of bases matching a transcript divided by the transcript length, still spanned six orders of magnitude (or five orders of magnitude when the five most highly covered transcripts were excluded).

To correlate vector integration and gene activity the arrays generated from patients' cells (THAL 22, 36, 37) and three healthy donors, activated by cytokines, we re-annotated the Affymetrix HG-U133 plus 2.0 probe sets with custom CDF files to include only probes unequivocally matching a transcript (Dai et al, 2005; Ferrari et al, 2007).

Although valuable, they do not specifically address the redundancy of probe sets but instead describe how well a probe set's constituent probes match a transcript (mRNA) record.

Genes (i.e. tags that matched a transcript) and tags that were only aligned to the genome were analyzed separately.

If off-target filtering is enabled, the foreground target site set is filtered to remove sites that contain a 15-nucleotide sequence from positions 6 20 (core target pairing sequence) that perfectly match a transcript that is not contained in the input set (background set).

Tags matching the known transcriptome were also categorised as matching a known transcript in the sense or antisense direction or matching multiple known transcripts.

Here, the proportion of reads matching a given transcript is used as a measure of its expression level.

Using TBLASTX with a cutoff criterion of E = 1e-10, 14,021 (40.6%) of the P. ultimum hybrid sequences matched a sequence within the three Phytophthora transcriptomes with only 945 (2.7%) of the P. ultimum sequences that align with a predicted Phytophthora protein not matching a Phytophthora transcript.