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There were slightly less similar matches to sequences classified in the Syntrophomonadaceae and Clostridiaceae families.
Regarding mutation, we found that the most abundant OTUs are generally composed of a unique RST with the highest numbers of tags characterized by exact matches to sequences in GenBank (100% if it is a known species), and a few other unique RSTs with lower numbers of tags and correspondingly lower matches to sequences in GenBank.
A total of 5921 short unigenes had significant matches to sequences in the NCBI nr database.
Therefore, for each target family we eliminated regions with perfect matches to sequences outside the target family.
A small number of markers show best matches to sequences that are on different chromosomes from where they map genetically.
We also found that 36 contigs had their best matches to sequences of four plant species including three crops (maize, rice and sorghum) and one grape species.
Similar(32)
Potential matches were seeded using perfect matches to sequence substrings.
Subsequent matches to sequence databases showed these features to be correct.
Genome similarity searches revealed sequence identity matches to sequence scaffolds on BTA6 for most unique ETS (91%).
Resulting sequences were matched to sequences in GENBANK via BLAST to determine the likely host species (98% sequence match).
About half of the sequences (19 unigenes) matched to sequences of unknown function and without known protein domains.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com