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Comparisons with N linkage showed that while basic E linkage resulted in few false positives (PPV of 97.7%) it missed nearly 20% of matches (sensitivity of 80.7%).
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In addition to having more longitudinal information, the upgrade plans include a new custom ADC with matched sensitivity and timing information.
With low quantities of culprit DNA (10 pg to 100 pg), computer-based quantitative interpretation provides greater match sensitivity.
Whereas quantitative modeling has a match sensitivity limit in the ten picogram range, the less informative qualitative inclusion sensitivity is in the hundred picogram range (Table 2).
A more proactive solution is to recognize the inherent information gap between the match sensitivity of older qualitative and newer quantitative interpretation methods.
A seed family is a collection of seed patterns that when used in conjunction provide improved matching sensitivity, and such families have been previously demonstrated to offer excellent speed and sensitivity [39].
Given the 100 pg match sensitivity limit for qualitative interpretation (Table 2), it makes sense for them to not expend considerable effort qualitatively analyzing DNA evidence that has low amounts of culprit DNA.
There is an order of magnitude information gap between the quantitative and qualitative interpretation methods, evident from the scatter plot data, regression lines, and match sensitivities.
This is a range of scales that matches the sensitivity of the method, as we do not expect to be able to accurately map tracts smaller than several milimeters in diameter, which is presently the size of our ROIs.
It matches the sensitivity of other techniques of RNA detection (i.e., northern blot) and is estimated to be approximately 1 2 copies per million of mRNA molecules [ 12].
Although such reads numbers are insufficient to detect changes in very lowly expressed genes (see Veneman et al, 2014 [ 49]) approximately 10 million total reads is currently a good cost efficient number that matches the sensitivity of microarrays [ 50].
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