Of the ∼1.98 million features, a total number of ∼1.87 million features matched a unique position in the genome.
Tiles have a melting temperature of 74°C on average and 88% of them match a unique position in the genome.
The sequence of fragment sizes thus generated should match a unique region of the optical map and provide a unique placement to the contig on the genome.
Furthermore, all but a few of the spike-ins were represented by multiple sequences, as many as 22, whereas virtually all Trinity assemblies matched a unique spike-in control, or produced at most two matches.
The number of times each 454 DNA sequence read matched a unique EST contig from the Ambystoma ESTdb was recorded, and these count data were used to estimate mRNA abundances for presumptive axolotl genes.
Similarly, paired-end reads where one read matched the internal Pong element sequence (23 5320 bp) and the mate matched to a unique genomic region of MSU7 were used to identify Pong insertions.
Paired-end reads where one read of a pair matched the internal sequence of a Ping element (253 5164 bp) and the mate matched to a unique genomic region of the Nipponbare reference genome (MSU7) were used to differentiate Ping insertions.
Two methods are typically used when trying to match people's information on one data set with their data on another: name-based matching (often probabilistic) or matching using a unique person identifier [ 23- 26], with data handling protocols being used to protect confidentiality and privacy [ 27, 28].
Last November, RealJukebox, one of the most popular Internet music players, with 30 million registered users, became a focus of media attention when privacy advocates noted that the player could relay information to its parent company, RealNetworks, about the music each user downloaded, and that this could be matched with a unique ID number that pinpointed the user's identity.
The DOC number was then matched to a unique individual's name and date of birth, and the resulting dataset was shared with University-based researchers.
