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The aggregated data was matched to reference chemical libraries to aid in identifying the ion set as a known metabolite or as a new unknown biochemical to be added to the library.
MiRNAs were the major component of small RNA libraries from rESCs being 60% of sequences, which were matched to reference miRNAs in each sample.
For detecting known miRNAs, clean reads were matched to reference miRNA precursor sequences in the rhesus macaque (miRBase version 16.0) using Blastall (-p blastn -F F -e 0.01).
Among the 7,764 matched ESTs, 1,439 could be matched to reference genes if the subjected starting site was set to be the first coding amino acid; meanwhile 2,325 matching genes could be found if the subjected starting site was allowed to be any of the first 10 amino acids.
Out of the tags matched to reference genes, approximately 10% were mapped to multiple locations, including low complexity tags with poly(A) tails and tags which derived from repetitive sequences and were mapped to highly conserved domains shared by different genes.
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Contigs thus ordered and those matching to reference sequence were joined together into a pseudochromosome, and non-matching contigs were added at the end in random order.
Placeholders are denoted in curly braces and it is possible to use pattern matching to reference multiple files at once.
Although the technology's sequence lengths are reduced compared with pyrosequencing, they sufficiently typify many microbiota in terms of sequence content and taxonomic assignments achieved by matching to reference databases that are also increasingly comprehensive.
For organisms with a reference genome, reads generated in the sequencing process are usually matched to the reference sequence with a variety of alignment algorithms.
The meters take an audio sample of the programme, which is then turned into a digital fingerprint and matched to a reference library of programmes.
Otherwise, additional narrow diffraction peaks were observed in the relatively low angle region, and the pattern was reasonably matched to the reference diffraction of 4HR (ICDD, 034-1558).
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