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Several genes were selected, including light-harvesting chlorophyll a/b binding protein (LOC_Os03g39610), starch and sucrose metabolism pathway related proteins such as glycogen/starch synthases (LOC_Os07g22930), 4-alpha-glucanotransferase (LOC_Os07g46790), and Glucan water dikinase (LOC_Os06g30310).The real-time RT-PCR results mostly matched the microarray expression patterns.
The method described above produced 737 transcripts that matched the microarray probes (E-value < 1).
For all 27 analyzed genes, the real-time PCR results matched the microarray data (additional file 1).
As shown in Supplementary Figure 2(A), the mRNA levels of RT-qPCR precisely matched the microarray results.
Of the 18 genes tested, the expression pattern for 12 genes completely matched the microarray data at four dpi time points.
Consistent with our microarray results, wherein several probes corresponding to C3AR1, CAMP, CCL5 and IFNγ exhibited a down regulation along with up regulation of C4BPA, real time PCR results also matched the microarray expression profile (Supporting Table S2 in Additional file 1).
Similar(51)
Since hypoxia, or more generally brutal variation in oxygen pressure, are considered as major actors of preeclampsia pathogenesis [23; 10; 24], it was interesting to match the microarray result with HIF1α induction.
The qRT-PCR data roughly matches the microarray data, implying that they are also 20E responsive.
For 15 out of 17 genes, the Q-PCR results match the microarray data.
However, 25% of expression patterns for which the qRT-PCR results did not match the microarray result.
This model matches the microarray data well but it is not satisfying enough in revealing more biological sense.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com