Sentence examples for matched the codon from inspiring English sources
The L-arabinose metabolic genes of Lactobacillus plantarum matched the codon usage of S. cerevisiae more closely than the genes previously reported.
Matching the codon usage of recombinant genes to that of the expression host is a common strategy for increasing the expression of heterologous proteins in bacteria.
It was previously engineered to match the codon usage in C. reinhardtii and was available on plasmid pPsaD-GLuc [ 33].
Furthermore, the use of synthetic genes matching the codon usage of the host microorganism can have a significant impact on gene expression levels and protein folding [ 14].
The luciferase (gluc) gene from the marine copepod Gaussia princeps, which previously was engineered to match the codon usage of C. reinhardtii, was used as a reporter gene.
A total of 57 codons were altered in the nS sequence to match the codon usage preferences of Nicotiana tabaccum, yielding an optimized-plant version of the SAG1 sequence named oS.
We also show that the luciferase (g-luc) gene from the marine copepod Gaussia princeps, which previously was engineered to match the codon usage of the unicellular alga Chlamydomonas reinhardtii, is a suitable reporter gene in V. carteri.
It does not appear that the prevalence of DSD is a result of an underlying primary nucleotide sequence preference, as the codons in DSD possessing proteins roughly match the codon preferences for each species.
A third approach to recode a target gene sequence is to "match" the codon usage bias inherent in the native host more closely when expressed in the heterologous host and is referred to as "codon harmonization" [ 62].
It is also likely that the kinetics of translation are improved by more closely matching the codon usage of a recombinant gene product to the usage of the expression host [ 3, 13- 17].
To investigate the efficiency of co-transformation in P. morum, three different plasmids were used containing the gluc gene of G. princeps, which was previously engineered to match the codon usage of C. reinhardtii[ 27].
