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Singletons contained a significantly higher proportion of sequences that best matched proteobacteria (P < 0.01) and amoebozoa (P < 0.05) records than reads that assembled into contigs, suggesting that singletons were enriched in cDNA from biological contaminants.
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There were significant differences among seasons in Proteobacteria (P < 0.001), Chloroflexi (P = 0.012), Actinobacteria (P = 0.014), and Alphaproteobacteria (P = 0.030).
The K n /K s values of mitochondrial genes are significantly lower than those for all proteobacteria (P = 0.013), as well as for all obligate proteobacteria (P < 0.001) and obligate alpha-proteobacteria (P < 0.001), but only marginally significantly lower than those for all free-living proteobacteria (P = 0.068; Fig. 2).
Oral TMS significantly reduced the relative abundance of Verrucomicrobia (P = 0.012), unclassified bacteria (P = 0.025) and a trend to reduce Proteobacteria (P = 0.052) and increased the abundance of Firmicutes (P = 0.012) after 5 days of treatment.
P: Wilcoxon matched test P value.
* p < 0.05 compared to time matched controls, ** p < 0.01 compared to time matched controls.
The free-living alpha-proteobacteria have higher K n /K s values as compared to free-living beta-proteobacteria (P = 0.003), and similar to those of free-living gamma-proteobacteria (P = 0.319; Fig. 2).
The K n /K s values for free-living gamma-proteobacteria are marginally higher than for beta-proteobacteria (P = 0.080; Fig. 2).
They are also inversely correlated when controlling for genome size for each clade (Spearman partial correlation: α-proteobacteria P = 0.044, β-proteobacteria P = 0.087, and Firmicutes P = 0.065; fig. 6).
The primate K n /K s values are larger than the glires ones (P < 0.001) and lower than K n /K s for obligate alpha-proteobacteria (P = 0.007).
Man of the match: K P Pietersen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com