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The relative expression between matched probes was examined.
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Positive probe pairs met two criteria: (1) the fluorescence of the perfectly matched probe was at least 1.3 times greater than the intensity of the control (mismatch probe); (2) the value of the difference between perfectly matched probe and mismatch probe intensities was at least 130 times greater than the squared noise value.
Ambiguous and poorly matching probes were discarded from the analyses.
Uniquely matching probes were used in the TMDH analysis, with their hybridisation position on the NCTC 8325 genome determined from the BLAST result.
Only perfect match probes were included in data analysis.
Only the perfect match probes were used to calculate model-based expression indices.
The unique and perfect match probes were picked up using MUMmer tool (http://mummer.sourceforge.net/).net/
Probes that could not be aligned to any gene and genes with no matching probes are reported.
For AFF analysis, only perfect match probes were taken into account and probesets were summarized with the "median polish" method.
The raw Affymetrix perfect match probes were normalized by the RMA method combined with median-polish [ 15].
Individual signals from the perfect match probes were pre-processed using the variant of the Robust Multichip Average algorithm which takes account of the probe GC composition [ 48].
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