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Finally, cross-species matched probe sets may be generated only for organisms with sufficient genomic data.
4. The generation of the cross-platform matched probe sets was based on unigene sequences.
This resulted in a list of clone pairs, which formed potato-microarray – tomato-microarray clone matched probe sets.
For the GO analysis, we matched probe sets to GO identifier using the information available in "biomart" (www.ensemble.org) version 36 for human genes (GRCh37).
These clone match probe sets were denoted as the unigene-based matched probe sets, and served for the construction of SEM (see below).
3. To facilitate extraction of SSH-like knowledge out of CSH, we have generated cross-platform (and hence cross-species) matched probe sets.
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According to the optimal genotyping criteria mentioned above, neither of the mutations CD17A>T or CD24T>A was genotyped to the fullest by the Tm matched probe set.
Utilizing the MSAW to apply the optimal stringency condition for each pair in the Tm matched probe set (method ii) showed that only two of the probe pairs for the nine mutations in the beta-globin gene functioned optimally at a sodium concentration of 17 mM (Figs. 4 and 5B).
Comparing the performance of a Tm matched probe set covering the nine investigated mutation sites revealed that only two conditions (17 or 42 mM Na+) allowed unambiguous genotyping of the entire set of samples at a single condition, and, of those two concentrations, 17 mM sodium gave slightly better results (i.e., superior separation of the genetic classes) (Figure 4).
Consequently, even the randomly matched probe set pairs tended to have similar expression patterns and thus low distances.
A number of maps of metabolic pathways were created by matching probe sets on the Vitis Genome Array to enzymes in known metabolic pathways (unpublished results).
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