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Notably, CD47 was the only gene upregulated in CTCs versus the matched primary tumours, indicating a survival advantage conferred by CD47 expression for peripheral blood CTCs106.
In general, metastatic PTC samples had stronger staining when compared with their matched primary tumours.
Interestingly, 20% (n=14) of the metastases showed a higher expression than matched primary tumours.
Next, we examined the LINE-1 methylation level in 26 matched primary tumours.
Microarray results indicate that it is possible to distinguish between the lymph node metastases and matched primary tumours.
Positive UBD protein expression was signicantly higher in metastatic colon cancer cells within lymph nodes than in matched primary tumours.
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The Beaumont Hospital cohort contained lymph node metastatic tissue for comparison with matched primary tumour tissue.
There was a strong positive correlation between TS nuclear expression in primary and metastatic CRC but the latter generally showed higher expression than matched primary tumour tissue.
Eight-millimetre trephine iliac crest bone biopsies containing metastatic prostate cancer were obtained from 14 patients from whom matched primary tumour tissue was also available.
Analysis of this information from a matched primary tumour and lymph node provides a clear picture of the presence or absence of a CR.
We did not find a significant difference in either CD68+ or CD163+ cell infiltration between the recurrences and their matched primary tumour samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com