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The Beaumont Hospital cohort contained lymph node metastatic tissue for comparison with matched primary tumour tissue.
We did not find a significant difference in either CD68+ or CD163+ cell infiltration between the recurrences and their matched primary tumour samples.
Eight-millimetre trephine iliac crest bone biopsies containing metastatic prostate cancer were obtained from 14 patients from whom matched primary tumour tissue was also available.
Analysis of this information from a matched primary tumour and lymph node provides a clear picture of the presence or absence of a CR.
There was a strong positive correlation between TS nuclear expression in primary and metastatic CRC but the latter generally showed higher expression than matched primary tumour tissue.
In contrast, there was a significant increase in Dicer expression when LN metastases were compared to their matched primary tumour (LN vs IDC+, p = 0.0202, Figure 2A).
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In general, metastatic PTC samples had stronger staining when compared with their matched primary tumours.
Interestingly, 20% (n=14) of the metastases showed a higher expression than matched primary tumours.
Next, we examined the LINE-1 methylation level in 26 matched primary tumours.
Twenty-eight liver and LN metastases showed a PIK3CA mutational status identical to that of the matched primary tumours.
Thirty-one liver and LN metastases showed an MSI status identical to that of the matched primary tumours.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com