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When including lower-quality matches (100% identity over at least 18 nt) the majority (77%) of spacers matched plasmids, suggesting that a major role for Salinispora CRISPRs is to defend against plasmid integration.
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Sixty-five percent of the group 1 spacers in CNH-941 and CNP-193 matched plasmids from Alphaproteobacteria, while the group 2 spacers in CNH-964 and CNP-105 equally matched phages and largely gammaproteobacterial plasmids.
However, none of the strain 504 SSH sequences matched plasmid sequences.
The two systems from R and F plasmids suggest that pAPEC-1 may be the result of recombination between R and F plasmids.
High copy numbers and stable maintenance of the pSci plasmids suggest efficient mechanisms for plasmid replication and partitioning.
In addition, only the 2000 human isolate contained a plasmid, suggesting plasmid loss or acquisition events.
Finally, our analysis revealed that most of the PAGs might be of plasmid origin suggesting that plasmid-plasmid gene exchange might be favoured in respect to phage-plasmid and chromosome-plasmid ones.
Some match sweaters, suggesting displaced sleeves.
For example, library 1 was derived from a mosquito line transformed with the plasmid pMos3DB2Her; six piRNA sequences were found to match pMos3DB2Her, but also two piRNAs matched the plasmid autoHermes.
Two particular inserts from the T4 genome allow high-frequency plasmid transduction, suggesting that each insert might contain a T4 replication origin.
Three-group comparison of factors used in matching suggests that matching was good.
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