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First, we found that Bcl-2 protein staining is strongly increased in gastric tumors specimens compared with matched peritumoral tissues.
Unsupervised principal component analysis (PCA) showed that the eight miRs signature discriminate the group of tumor samples from that of matched peritumoral tissues.
We interrogated simultaneously the expression of 851 human miRs of 39 GC specimens and of their matched peritumoral tissues using the Agilent microarray platform.
In the present study, we performed genome wide analysis to interrogate miR expression profile of gastric tumor specimens compared with their matched peritumoral tissues.
Our analysis revealed that miR-204 is consistently and statistically (P-value= 5.34*10−7) downregulated in a significant manner in gastric tumor specimens compared with matched peritumoral tissues from all the three analyzed subsets.
Among those miRs, we identify miR-204 that is statistically significantly downregulated in GC samples derived from three independent subsets of patients (n=123) compared with their respective matched peritumoral tissues.
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To select the cutoff value, we calculated the P-value in log-rank (LR) survival analysis at each score of HSF1 and MCT4 expression in tumour and matched peritumoral tissue.
Formalin-fixed, paraffin-embedded tumor samples from 239 NSCLC cases and 123 matched peritumoral tissue specimens were collected in NSCLC patients from the Department of Pathology, the Affiliated Hospital of Nantong University from 2004 to 2009.
Hepatocellular carcinoma and matched peritumoral liver tissues (n=332) were semiquantitatively analysed for HSF1 expression, followed by correlation with clinicopathological parameters (patient outcomes).
Preliminary findings suggest that 9q21.12 chromosome portion, where miR-204 is located within intron 6 of human TRPM3, is selectively deleted in tumoral tissue when compared with matched peritumoral samples.
Then, tissue microarray sections with 180 pairs of tumors and matched peritumoral samples were constructed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com