The cancer tissues and matched normal tissues were procured from Tissue Procurement Core at Washington University Siteman Cancer Center (St .Louis, Missouri, United States).
Here, we found that miR-33a exhibits a decreased expression level in breast cancer tissues compared with matched normal tissues.
(B) In situ hybridization analysis of miR-33a expression in human breast cancer tissues and matched normal tissues.
(A) The expression of miR-27b in human gastric cancer tissue samples and matched normal tissues from 19 gastric cancer patients was analyzed by real-time PCR.
As is shown in Fig. 2A, miR-27b expression was significantly decreased in gastric cancer samples compared to matched normal tissues.
Human colon cancer tissues and matched normal tissues have been used by our laboratory recently [10].
Several reports have demonstrated that SEL1L protein expression varies in human tumors relative to matched normal tissues, suggesting an involvement in cancer progression [27] [32].
Using tiny amounts of biological samples we identified phage-Abs binding to receptors preferentially expressed on primary tumor cells rather than on cells obtained from matched normal tissues.
Additionally, significant upregulation of WISP-1 was found in the cancer samples (P<0.001) in comparison with matched normal tissues (Figure 2).
Total RNA was isolated from tumor and matched normal tissues, renal cancer cell lines using RNeasy Mini Kit (Qiagen, Netherlands) according to the manufacturer's instructions.
All the cases had matched normal tissues.
