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Sections were pretreated with 0.03% H2O2 for 20 minutes to block endogenous peroxidase activity and incubated in matched normal sera (Vector Laboratories, Burlingame, CA).
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The second set consisted of 45 sera from breast cancer (BC) patients and 46 matched normal women (CON), collected between January 2003 and July 2005 (referred to as the BC set).
All the cases had matched normal tissues.
In groups 2 and 3, samples from patients with cancers, matched normals, benign lung disease and control sera for the assay were interspersed in the order samples were assayed so that any batch effects would be spread over all sample types.
OA and normal sera were matched by age, sex, and BMI.
Spots on immunoblotting maps were matched to the reference map, and those detected in early HCC serum but not in liver cirrhotic or normal sera were excised for protein identification.
Substantial apoptosis was detected in EPCs after culturing in the presence of scleroderma sera compared with normal sera.
ELISA for anti-ATGAM antibodies was negative in testing 46 control patients and pooled normal sera.
In contrast, none of the six normal sera from apparently healthy individuals reacted with this protein.
The vast majority of normal sera, showed no or low reactivity with optic nerve proteins.
These melanomas, with CD7-positive inflammatory cell infiltrations, frequently highly express SECTM1, including an N-terminal, soluble form, which can be detected in the sera of metastatic melanoma patients but not in normal sera.
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