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Eighteen primary colorectal cancers were collected with matched normal mucosal samples.
Three CRCs and matched normal mucosal tissues were used for label-free quantitative analysis.
CRC and matched normal mucosal tissues were obtained during surgical resection.
Among the 4 proteins, HSPA8 can identify CRC from matched normal mucosal with the highest accuracy.
Primary tissue cultures were established using tumor tissues and matched normal mucosal tissues collected from four laryngeal cancer patients.
Paraffin-embedded sections (2.5 μm) of human CRC and matched normal mucosal tissues were collected on gelatin-coated slides.
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Primary tumors were compared with non-matched normal mucosal samples.
Gastric mucosal frozen specimens of matched normal volunteer and PHG patients with cirrhosis were obtained from the endoscopic unit of The Third Affiliated Hospital of Sun Yat-Sen UniverSun Yat-Sen
All the cases had matched normal tissues.
The samples were collected according to the following histological prerequisites: the CRC tissues had more than 80%% tumor cells; the matched adjacent normal colorectal mucosa tissues had normal mucosal structure without dysplastic cells.
Two inclusion criteria were used to select the subjects: (i) the CRC tissues should have more than 80% tumor cells and (ii) the matched adjacent normal colorectal mucosa tissues should have normal mucosal structure without dysplastic cells [ 13].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com