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In contrast, we found that the expression of both TET2 and TET3 as well as the upstream genes IDH1 and IDH2 were significantly decreased in human HCC tissues as compared to matched "normal" liver tissues (Fig. 2a).
Eighteen tumour and five matched normal liver specimens were supplied by the Tissue Bank of the Japanese Study Group for Pediatric Liver Tumour (JPLT) (Matsunaga et al, 2004), and 36 were supplied by institutions affiliated with Saitama Cancer Center.
The change for mRNA in HCC tissues relative to the matched normal liver tissues was calculated using the 2−ΔΔCt method, where ΔΔCt = ΔCt HCC/normal liver tissues and ΔCt = CtmiR-20b − CtGAPDH.
Moreover, in another study by Yang et al., the decrease of all three TET genes was shown in three pairs of frozen human hepatocellular carcinoma tissue compared to matched normal liver tissue [ 27].
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To investigate whether KDM5C might be involved in HCC, the mRNA expression level of KDM5C in HCC tissues and its matched normal adjacent liver tissues was determined by qRT-PCR in 18 samples.
miR-1 expression is significantly reduced in primary human hepatocellular carcinomas (HCCs) compared with matching normal liver tissues [ 39].
All the cases had matched normal tissues.
Ten age-matched healthy volunteers with normal liver enzymes and synthesis served as controls.
In this study, the expression patterns of all 19 WNT, 10 FZD, two LRP, and four sFRP genes have been determined in HCCs and in their matched surrounding precancerous peritumorous areas, by comparison to normal liver (NL) tissues.
In our study, we have considered several reliable ultrasound parameters to define echogenicity of the liver and we defined hepatomegaly in reference to normal liver size in age and sex matched children [ 13].
Forty-three healthy blood donor volunteers matched for age and sex were recruited as controls: normal liver function, no signs of fatty liver on ultrasound and negative serology for viral hepatitis.
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matched noncancerous liver
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