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All four Tensins were found to be highly significantly lower or absent in RCC tissue versus matched normal kidney cortex tissue.
Description of hypermethylated and hypomethylated differentially methylated regions (DMRs) in nephrogenic rests compared with matched normal kidney.
BGv1 was expressed in all RCC samples and the levels were significantly elevated compared to the matched normal kidney tissues.
As expected from the cell line data, miR-584 expression level was also significantly lower in primary ccRCC tissues than in matched normal kidney tissues (n=14).
We demonstrate the use of our method on expression data from clear cell Renal Cell Carcinoma (ccRCC) and matched normal kidney samples.
Quantitative real time RT-PCR (qRT-PCR) analysis revealed the normal expression of HIF-1α, PHD2 and significantly high expression of PHD3 mRNA (3 out of 4) in primary tumors compared to their matched normal kidney.
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We also checked KKv1 expression in matching normal kidney of the 46 cases and found that only ten had detectable levels of KKv1.
A total of 27 nodules representing different time points of tumor progression (Table S7 ) were collected from 10 Mdr2 −/− mice (7 males + 3 females) sacrificed at 13-16 months, together with matched normal tissue (kidney).
SLIT2 promoter methylation was also detected in one out of 12 of the matching normal kidney tissue samples for methylated tumours.
OXPHOS and glycolysis have been evaluated in numerous tumor types, including renal clear cell carcinoma (RCC) in which the majority of glycolytic enzymes are overexpressed and mitochondrial enzymes underexpressed relative to patient-matched normal kidney cortex [6].
Young et al. hybridized RNA from seven tumors and seven patient-matched normal kidney tissue samples to a 7,075 element cDNA microarray [ 12].
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