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Figures 5G and H show decreased immunostaining for collagen type IV, a ubiquitous component of basement membranes, in donor ONH tissue from AA compared to an age matched normal CA donor.
Employing an exhaustive series of pairwise class comparisons between age-matched normal donor samples (≥ 60 years) and the five graded AMD/pre-AMD phenotypes from the Iowa cohort, we identified numerous candidate AMD-associated genes, all of which satisfy a minimum fold change of 1.5 and permuted P-value cutoff of 0.1 (Table S1 in Additional file 2).
RNA from 13 AA and 17 CA age-matched normal donors was used to validate microarray data.
In this study, we have identified several sites of histone PTMs in frontal cortex from human donors with AD that are differentially abundant compared to age-matched normal donors.
As there was no normal donor tissue used in this study, it was not clear whether the absence of a difference in SOCS expression between tumour and matched normal background tissue in our cohort was due to the effect of field cancerization.
All the cases had matched normal tissues.
METHODS: Human RPE cells were harvested from normal donor eyes and propagated in culture.
The level of up regulation appeared to have some normal donor –to- donor variation.
Figure 3a shows the KIR repertoire of a normal donor.
Seven normal donors age-matched with the patients (median age 73 years; range from 61 to 80 years) as well as 12 normal donors recruited from among the laboratory personnel (median age 33 years; range from 30 to 49 years) were included in the study.
We used oligonucleotide Affymetrix microarray (HG U133A & HG U133A 2.0 chips) to compare gene expression levels in cultured ONH astrocytes from twelve CA and twelve AA normal age matched donor eyes.
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