Expression in lung cancer cell lines was analyzed relative to the non-cancerous immortalized lung epithelial cell line Beas-2B [ 21].
To determine the expression levels of these oncogenic proteins in NSCLC cells, western blotting analyses were carried out in various NSCLC and immortalized lung cell lines.
Using immortalized lung fibroblasts and podocytes isolated from homozygous Actn4 KI mice, Weins et al. observed that mutant Actn4 aggregates with F-actin [ 60].
HPL1D is an immortalized lung epithelial cell-line that is growth inhibited by TGF-β, similar to many epithelial cells [ 15].
*p < 0.05, **p < 0.01 To examine the role of CIP2A in lung cancer, we first established the expression profile of CIP2A in two immortalized lung cell lines (BEAS-2B and HBEC3), six lung cancer cell lines including four non-small cell lung cancer cell lines (A549, H460, H1299 and H838), and two small cell lung cancer cell lines (H69 and H146).
Surprisingly, this gene was not identified as up-regulated in previous adhesion experiments performed in our lab [ 9], and it even showed a slight, although not significant, down-regulation when A. pleuropneumoniae adhered to immortalized lung epithelial cells.
A recent study has shown that a gene-specific promoter methylation is induced in immortalized lung epithelial cells after prolonged (several months) exposure to cigarette smoke condensate (Liu et al., 2010).
In both human lung adenocarcinoma lines (H1838 and H322 cells) and immortalized lung epithelial cells (BEAS2B), the expression of NICD1 increased SOX9 (sex determining region Y-box 9), a transcription factor promoting EMT, migration, and invasion of lung adenocarcinoma cells [ 35].
Tumor and matched normal lung specimens of each patient were stored in the hospital tumor bank.
On comparing men and women matched for lung size, women had significantly smaller airway luminal areas.
