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To determine whether the secretory patterns we observed matched gene expression trends we investigated M. tuberculosis -induced mRNA transcripts in stimulated PBMCs by studying CCL2 and CCL3 expression.
Further, we examined their transcript levels using matched gene expression data for androgen-treated LNCaP cells (see Materials and Methods section).
These observations lead us to the validation of this finding at multiple levels; starting at the level of regulatory controls with a combination of matched gene expression and chromatin immunoprecipitation assays.
The matched gene expression data was processed as described previously [ 26].
Next, Figure 2 shows the inverse correlation between DNA methylation and matched gene expression of MGMT.
We used the Illumina 450 K platform to evaluate DNA methylation patterns of 50 metastatic melanoma tumors, with matched gene expression data.
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In addition, we included data from 52 tumors from our previous genomic analysis of platinum-resistance in ovarian cancer [3] and obtained matching gene expression data for all samples [17].
If we consider a gene model significantly changed if it had at least one flag in the FDM sequence that was successfully bridged and aligned to scaffold, a change in the total number of exons, and/or an absolute change in expressed gene size of more than 10%, then of the set of 1439 aligned BESs, 974 (69%) of gene models required changes to match gene expression.
At the same time, the apparent protein surface abundance did not closely match gene expression data for several of the targets.
Multiple methods based on matching gene expression signatures have been proposed to identify anti-correlated drug and disease profiles (Hu et al., 2009; Sirota et al., 2011).
The user can retrieve miRNA target genes from six different databases, run pathway enrichment analysis for the targets, and correlate miRNA expression with matching gene expression data if available.
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