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There were also no internal baseline differences in metaphyseal or diaphyseal bone morphology between left and right hindlimbs of age matched control mice and, therefore, the average of the two limbs for any given index was used for further analysis.
Genotyping knockout and age matched control mice showed that the ΔTrsp allele (450-bp product) was detected in skin and tongue of both genotypes, while ΔTrsp allele was observed in the thymus of knockout mice (Figure 2A).
We found no visible differences in the Dlc1gt/+ mice mammary glands compared to the age matched control mice [Fig. 5a and b].
GAPDH was used as the endogenous control, and gene expression of target genes for infected samples were quantitatively measured relative to those of RNA from mock-infected, age matched control mice.
No significant differences in body weight were found in HU mice compared with age matched control mice during all the experimental periods, nor with mice kept in HU for 14 days plus 14 days after reloading of hind limbs.
Intravitreally injected HYPOX-1 did not accumulate in age matched control mice, which develop fully vascularized retinas, and lack of Pimonidazole adduct staining was also confirmed in these retinas.
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Parametric comparisons between thickness values of age-matched control mice and knockout mice were made by unpaired two-tailed Student's t-test.
This increase also appeared at the same ages doubling or almost doubling the values from age-matched control mice (227%, P=0.0148 at 3 months, and 180%, P=0.0329 at 18 months; Figure 4b).
Mechanical ventilation was associated with increased ERK1/2 phosphorylation in lungs of both WT and Tpl2-/-mice compared to genotype-matched control mice, *p < 0.05.
BALF IL-6 was lower in genotype-matched control mice than in ventilated mice, *p<0.05.
Animals used were 6 13 week-old male Prkg1SMr mice (n = 10) and litter-matched control mice (n = 21), and 10 36 week-old male Prkg1BKO mice (n = 8) and litter-matched control mice (n = 8).
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