Sentence examples for matched control IgG from inspiring English sources

No staining was detected with isotype matched control IgG (Fig. 1A, right).

Antibodies against β-tubulin and Muc5AC (Santa Cruz Biotechnology, Santa Cruz, CA) and matched control IgG were used for immunocytochemistry.

In all cases, an isotype- matched control IgG was used as a negative control.

To verify whether the Ozz-MyHCemb interaction occurred in vivo, crude lysates of proliferating (day 0), differentiating (day 2) and terminally differentiated (day 4) primary myoblast cultures prepared from newborn wild-type mice were immunoprecipitated with anti-MyHCemb antibody or an isotype matching control IgG, and probed on immunoblots with anti-Ozz antibody.

Frozen sections (5 µm) of gastric tissue were fixed with 4% Cytofix/Cytoperm (BD Pharmingen) and incubated with blocking buffer (Perkin Elmer) for 30 min at room temperature, followed by primary antibodies or isotype-matched control IgG.

DRBP76 antibody, but not mouse isotype-matched control IgG, efficiently precipitated the previously observed distinct immunoblot signals from HEK293 cell- and primate brain cytosolic extracts, and yielded co-IP of NF45 in both instances (Fig. 3A, left panel).

TGC-macrophages (2×105 cells/well in 96-well flat-bottom plates) were incubated with 0 100 ng/ml LPS (Salmonella enterica serotype typhimurium; SIGMA) in the presence and absence of 40 µg/ml anti-ST2 mAb, anti-IL-33 mAb or isotype-matched control IgG for 24 or 48 h.

MCs (2×105 cells/well in 96-well flat-bottom plates) were cultured with 1 µg/ml IgE (SPE-7, Sigma), 30 or 100 ng/ml rmIL-33 (R&D Systems) and a combination of 1 µg/ml SPE-7 plus 100 ng/ml rmIL-33 in the presence and absence of 40 or 80 µg/ml anti-mouse ST2 mAb, anti-IL-33 Ab or isotype-matched control IgG for 24 h.

Isotype-matched control IgG (Becton Dickinson) served as negative control.

Isotype-matched control IgG was used for control staining.

Cells were stained with the corresponding isotype-matched control IgG (BD Pharmingen, San Diego, CA, USA).