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The reciprocal changes in behavior of these matched cell lines cross-corroborate each other.
They also allow researchers to make genetically matched cell lines from patients.
IC50s were similar in matched cell lines that did and did not express Pgp1.
In addition, a cell line derived from a parent of an FD patient, and therefore heterozygous for the FD mutation, and four matched cell lines derived from healthy individuals were used as controls.
Comparison of exactly matched cell lines and cultures could assess the association between them more accurately.
The matched cell lines were then differentiated into midbrain dopaminergic (mDA) neurons.
Similar(40)
Copy number heterogeneity from a tumor was represented in matched cell line clones, which also varied in their clonogenicity and drug sensitivity.
To assess the presence of bias introduced by data normalization, log2 ratios for each matched cell line and xenograft pair were calculated for 56 miRNAs that were assayed and detected by qPCR.
In support of this assertion, a ∼ 22-fold differential in clonogenic LC90 values was found between L1210 cell lines expressing and not expressing MGMT for 90CE, compared to only a ∼ 2-fold differential for BCNU in the same matched cell line pair.
Besides encapsulation, other options to avoid a devastating immune reaction would be to generate banks of matching cell lines (so-called "haplobanks") or to engineer the genome of cells to cause less response from the host by for example knocking out immune-reaction causing antigens[21].
Using the HLA type, seq2HLA provides a sample annotation quality control, both for previously un-typed cell lines and for matching cell lines with previously determined HLA types [ 34].
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