During this procedure, amount of experimenter contact and duration of separation from the dam were matched between Novel and Home pups, isolating exposure to a novel environment as the critical difference between the two groups.
RPE cells were isolated from mouse pups at post-natal days 9 to 14 and were age-matched between wild-type and knockout mice.
We compared global DNA cytosine methylation as well as DNMT1, DNMT3a, and DNMT3b expression levels in hippocampal pyramidal layers of male P3 pups born to cocaine-treated females compared with matched pups from saline-control dams.
Each of 2 unweaned pups matched their mother's serostatus, with 1 pup positive by SNT (pup 80, mother >640).
Thus, the vocal similarity between pups and harem males wasn't because of genetic relatedness.
Several spectral features of syllables changed between pups and adults.
We also compared the overall difference in entropic change between pups younger than p13 and adults, and between p13 pups and adults; adults showed a greater degree of higher-order structure than pups of any age.
The mixed model includes a random litter effect, which models the correlation between pups within a litter and allows the pup to be the unit of analysis.
Statistical comparisons were made by the use of instat software, between pups born to mothers injected with vehicle and pups born to those treated with Ro61-8048.
Such information will allow the in-depth studies of the link between PUPs and diseases.
