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Only the perfectly matched alignments for the given read were kept and extended to 35 nt to 36 nt by adding 15 nt of upstream sequences.
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For the first case, the fully matched alignment without splicing (3.a in Fig. 1), which is termed 'exonic alignment', only extends from one seed.
All uniquely matching alignments were retained.
Such "close match" alignments may be considered problematic since they represent potential mis-primings.
However, with increase of mismatches (-v) and multiple alignments (-m) in Bowtie1 options, miRNA precursors identified by eRNA covered almost all precursors identified by miRspring and miRDeep2, due to the different considerations of these tools on multiple or non-exactly matching alignments.
Of the 19,117 Arabidopsis genes matching alignment assemblies, only 2,867 (15%) lack a FL-cDNA match.
In order to perform matching, alignment of DNA sequences and searching for putative SNPs, the SSAHAsnp Program (Sequence Search and Alignment by Hashing Algorithm) was used [ 65].
Non-matching protein alignments coming from matching nucleotide alignments were considered as genes with alternative reading frames.
The slider alignment is based on two steps of exact match alignments: The first step is to align the P0_Reads table with the reference database table.
Descriptions of the probe to genome alignments are stored in the database as either perfect 'Full match' alignments or 'Mismatch' alignments for those probes that align with only a single base pair difference between the probe and the genome sequence assembly.
Structural matching, alignments, ribbon figures, capsid predictions and root mean square deviation (RMSD) data were produced using Chimera [55].
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