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We also compared miR-125b expression levels in a series of 36 pairs of gastric cancer tissues and their matched adjacent tissues.
In this study, we demonstrate that expression of microRNA-124 (miR-124) is significantly downregulated in colorectal cancer tissues and cell lines, compared to the matched adjacent tissues.
As shown in Figure 1B, the expression of miR-7 significantly reduced in NSCLC tissues relative to their matched adjacent tissues (P=0.0031).
In summary, differential methylation of imprinted genes is a frequent finding in a series of HCC samples, relative to their matched adjacent tissues.
DNA methylation alterations have been studied extensively in breast tumour tissues most recently with genome wide analyses revealing hypermethylated as well as hypomethylated loci compared with matched adjacent tissues (14).
In this study, we found that the expression of miR-7 was significantly downregulated not only in NSCLC cell lines, but also in human NSCLC tissues compared with the matched adjacent tissues.
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In ex vivo tissue the possibility of cross staining endogenous E. coli was excluded by ensuring that matched, adjacent tissue (from the same patient) was completely negative for E. coli via immunofluorescence.
The HCCs had less PCSK9 mRNA when compared to matched adjacent tissue, although this did not reach statistical significance (p-value = 0.08).
Among these 79 patients, 18 patients with tumor and matched adjacent tissue specimens were firstly applied for the examination of miR-224 expression.
GC tissue microarray chips with follow-up data (No. HStm-Ade178Sur-01) containing 67 GC tissues paired with matched adjacent normal tissues, 39 gastric malignant tissues, and 5 adjacent tissues were obtained from Shanghai Biochip Company.
Similar to the results of qRT PCR in the corresponding tissues, EphA5 protein level in prostate tumour samples was significantly lower than that of matched adjacent normal tissues or BPH tissues.
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