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Exact(4)
Detailed analysis of unique gene reads (number of sequence reads that match uniquely to the genes) and unique exon reads (number of sequence reads that match uniquely to the exons, including exon-exon junctions), revealed a threshold value of 0.2 RPKM for detectable gene expression in MSC [ 18].
Only probes that match uniquely to the genome and to at least one transcript (or overlapping transcripts) are retained for further analysis.
We expect sufficiently large sequences with many restriction sites to match uniquely to the optical map.
In each plot, probes are partitioned into two groups those probes which match uniquely to the target transcript and those probes which match to the corresponding off-target.
Similar(56)
DAVID found an additional 104 significant GO-categories uniquely associated with the CI-derived gene list, while the RMA-derived list matched uniquely to 63 additional significant GO-categories (supplemental data, Table S3).
Both Filter 1 and Filter 2 are conservative and the reads after the filtering all matched uniquely to the Entrez gene database (could be more than one RefSeqs due to alternative splicing).
Probes matching uniquely to the gene Scd3 show a different expression pattern than the probes matching Scd1, whereas for the gene Scd1, the probes either matching uniquely to Scd1 or those which also match Scd3 show the same expression patterns.
Among such good probes, which formed 63% of the total probes, 68% matched uniquely to a specific transcript.
Data from the STI clinic were all matched uniquely to the register based on the whole name and date of birth.
Between 87.3% and 92.1% of the reads in a given RNA-Seq run matched uniquely to the 10403S pseudochromosome and thus were used in subsequent analyses.
However, in the case of pure datasets, still a significant number of spectra is matching uniquely to the absent species (about 15%).
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Justyna Jupowicz-Kozak
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