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Where possible, putative functions were assigned by BLAST match to sequences deposited in Genbank.
The other three genes have no match to sequences from non-mosquito taxa, thus can be regarded as orphans.
Long (>15 nucleotide) primers match specifically with a designed target sequence, but they can also match to sequences that are complementary to shorter portions of the primer.
Furthermore, the match to sequences in the EST databases of rice indicated the largest region of significant homology (94% identity to rice EST CK043734) was only 208 bp and thus it seems unlikely to be an active entity.
Among the matched unigenes, 12.8 % has a best match to sequences from Ricinus communis, followed by Populus trichocarpa (10.7 %), Glycine max (5.9 %), Medicago truncatula (2.1 %), Nicotiana tabacum (2.0 %), Solanum lycopersicum (1.8 %), and other plant species.
Among the BmiGI members which had Blast hits, BSR analysis found approximately 2,300 R. microplus sequences which did not have a close match to sequences from D. melanogaster, H. sapiens, C. elegans, or A. gambiae.
Similar(50)
Of the three chiRNAs used to generate germline transformants, only the Y5′ chiRNA has a 12-nt match to sequence adjacent to a PAM elsewhere in the genome.
There were slightly less similar matches to sequences classified in the Syntrophomonadaceae and Clostridiaceae families.
Resulting sequences were matched to sequences in GENBANK via BLAST to determine the likely host species (98% sequence match).
About half of the sequences (19 unigenes) matched to sequences of unknown function and without known protein domains.
More than 20 million 25-base 5'-end tags were obtained from untreated and 5Aza-treated cells and matched to sequences within the human genome.
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CEO of Professional Science Editing for Scientists @ prosciediting.com