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Unlike macroscopic valve designs, the protein allows differential ion flows in the pumping and resting states that are tuned to match the physiological timescales of the cell, thus creating a "kinetic" valve.
The aim of this study was to identify interacting eukaryotic proteins of YopM under conditions which match the physiological situation during infection as closely as possible.
In vitro experiments using purified proteins and controlled substrate concentrations to characterize enzyme kinetics are conducted under conditions that do not necessarily match the physiological environment, but are determined based on a number of factors, including the requirements of the assays used to measure the kinetics.
All experiments were performed at 37 °C to match the physiological temperature.
Upregulation of the functional beta cell mass is required to match the physiological demands of mother and fetus during pregnancy.
These findings reveal that clock-controlled gene expression is tailored to match the physiological role of the tissue.
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The ratio [ATP]/[ADP] = 10 has been used throughout the calculations, approximately matching the physiological ratio.
The first expansion produces higher compliance than the second, as expected, and matches the physiological knowledge.
Protein degradation in normal living cells is precisely regulated to match the cells' physiological requirements.
Large, nonphysiological bulk rises in Ca2+ can activate NFAT, even in the absence of AKAP79, by raising cytoplasmic Ca2+ sufficiently to match the local physiological Ca2+ rise near Orai1.
Metabolite-responsive repressors of human-pathogenic bacteria are particularly attractive for use in these orthogonal synthetic mammalian gene switches because the trigger compound sensitivity often matches the human physiological range.
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