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It was previously engineered to match the codon usage in C. reinhardtii and was available on plasmid pPsaD-GLuc [ 33].
The luciferase (gluc) gene from the marine copepod Gaussia princeps, which previously was engineered to match the codon usage of C. reinhardtii, was used as a reporter gene.
To investigate the efficiency of co-transformation in P. morum, three different plasmids were used containing the gluc gene of G. princeps, which was previously engineered to match the codon usage of C. reinhardtii[ 27].
We show that a Gaussia luciferase gene variant, engineered to match the codon usage in the Chlamydomonas nuclear genome, serves as a highly sensitive reporter of gene expression from both constitutive and inducible algal promoters.
A total of 57 codons were altered in the nS sequence to match the codon usage preferences of Nicotiana tabaccum, yielding an optimized-plant version of the SAG1 sequence named oS.
We also show that the luciferase (g-luc) gene from the marine copepod Gaussia princeps, which previously was engineered to match the codon usage of the unicellular alga Chlamydomonas reinhardtii, is a suitable reporter gene in V. carteri.
Similar(47)
The anticodon of 28 tRNA genes match the codons of a total of 14 amino acids.
Matching the codon usage of recombinant genes to that of the expression host is a common strategy for increasing the expression of heterologous proteins in bacteria.
Furthermore, the use of synthetic genes matching the codon usage of the host microorganism can have a significant impact on gene expression levels and protein folding [ 14].
The L-arabinose metabolic genes of Lactobacillus plantarum matched the codon usage of S. cerevisiae more closely than the genes previously reported.
It is also likely that the kinetics of translation are improved by more closely matching the codon usage of a recombinant gene product to the usage of the expression host [ 3, 13- 17].
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