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To improve utility of these data in preparation for ASGARD deposition, the O. fasciatus and P. hawaiensis transcriptomes were further annotated to match the annotation status of the G. bimaculatus transcriptome (V.
Then only those reads that did not match the annotation were used as input for Cufflinks.
True positives were nucleotides of exons with a prediction that matched the annotation, whereas false positive regions were predicted as exons by the gene-finding method, but did not match the annotation.
For the novel assembly the mapped reads were split, using split_bam.py from RSeQC [ 31], into two bam files; the reads that matched the annotations and the ones that did not match the annotation.
For assessing annotation metrics, we adapted the standard definitions of recall and precision, defining precision as the percentage of annotations made from Textpresso sentences that exactly match the annotation in the gold-standard set and recall as the percentage of annotations made from Textpresso sentences that either exactly match or are a parent term of the gold-standard annotation.
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MiRBase::Targets version 4 was used to match the annotations used in constructing the Agilent human miRNA array (G4470A).
A putative homolog from the first search is classed as a homolog if one of the closest matches in the second search matches the annotation of the query sequence [31], [32].
For dUTP libraries the first read in a read pair must be reversed so it matches the annotation while the second read matches the annotation directly.
Several enzymes showed activity on multiple substrates, but in most cases the highest measured activity matched the annotation of the enzyme.
Here, strand specificity means the percentage of times the read matches the annotation correctly according to how the library was made.
Of the 1401 pairs of clones from this abiotically stressed leaf library, 114 (7%) pairs matched the annotation of different genes and the ID with greatest confidence score was retained.
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