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Recently, several algorithms have been published that match reads more directly to the genome, including SplitSeek [23], SuperSplat [24], and SpliceMap [25].
Furthermore, for exact match (0-mismatch), several orders of magnitude more reads map to ESJ than to ERJ regardless of the MMES scores, which implied that exact match reads were the most reliable to detect spicing junction, no matter which part of the junction the read was aligned to.
NeXtype ranks these EAGs by score, i.e. number of "best match" reads in descending order.
Only EAGs that score at least 5 "best match" reads are subsequently considered.
Among them, the number of unique match reads ranged from 7.0 7.9 million (Table 2).
Instead, we relied on all Perfect and Best Match mappings of the reads but excluded all Common Match reads.
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His career Test record after that match read: 77 wickets in 23 Tests at an average of 34.09.
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Across all SSPACE assemblies only 1 1.8% of the MP read pairs satisfied the perfect match, read orientation, and insert size criteria (Table 3).
If there is no exact match, read from a scale that's an easy multiple of the setting.
(2) exact barcode matching, two nucleotide mismatch in primer matching, reads containing ambiguous characters were removed.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com