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To match previously identified haplotypes, the sequences were aligned with known haplotypes of D. vexillum available from GenBank (Stefaniak et al. 2009, 2012).
In order to identify sequence reads that match previously identified miRNAs, we aligned sequences against miRNA data from miRBase release version 10.1 [ 6] using BLAT [ 75]. miRNAs with varying 3' terminal were grouped together for tag counts.
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We identified 14 putative loci from individuals from Easter Island and Guam, and most matched previously identified loci of individuals of C. miliaris from American Samoa [10].
We only considered sequences that perfectly matched previously identified mature miRNA sequences as known miRNAs.
A total of 3758 sequences in our library matched previously identified miRNAs listed in miRbase ('conserved miRNAs' in Figure 1B).
A further 76 sequences (5.8%) matched previously identified coding regions of unknown function and 445 sequences (34%) resulted in no match with annotated sequences in any of the public databases.
A total of 19 different ribotypes were identified, including 5 ribotypes that matched recognized international designations, 4 that matched previously identified ribotypes in our laboratory (including four that have been previously identified in humans and 1 that was detected in a raccoon in 2010), and 10 that have not been previously identified in our laboratory.
Furthermore, the six most frequent chromosomal pairings in our study, all matched previously identified chromosomal homologies, with 13 out of the 15 most frequent chromosomal pairings observed here also being identified in previous studies (Phillips et al. 2009; Lien et al. 2011) (fig. 6).
Motif 2 does not appear to match any previously identified S. meliloti promoter motifs or any motifs in the manually curated prokaryotic PSSM database, RegTransBase [ 81].
22,158 coral sequences had significant matches in nr with an e-value ≤ 10-4; these represent sequences with reasonably strong matches to previously identified proteins in other organisms.
We also identified numerous other matches to previously identified subfamilies of the Ya-lineage (Shen et al. 1991; Roy et al. 2000; Jurka et al. 2002) summarized in table 4. A more refined illustration of the subfamily evolution within our Ya5 elements is shown as figure 4 C.
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