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To adjust for the small variability among ELISA plates, one standard sample of tortoise plasma, serially diluted to match experimental samples, was run on each ELISA plate.
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The S signal is the number of nanograms of the reference DNA that matches the experimental sample for copy number of the single copy gene.
The T signal for an experimental DNA sample is the number of nanograms of the reference DNA that matches the experimental sample for copy number of the telomere repeats.
The T/S ratio was determined from the average quantity of reference DNA found to match with each experimental sample for the copy number of the targeted template (the number of telomere repeats for T and the number of beta-globin gene copies for S).
3) All reference and cell line sample data are analyzed simultaneously to identify the best match for each experimental sample.
To go further, we then calculated the values of Δn necessary to match experimental and calculated intensity levels for the different samples previously considered.
The model predictions – including time to ignition and initial, peak, and rate of rise of sample mass loss rate – were found to closely match experimental results.
Image processing (contrast enhancement) was equally applied to all matched experimental and control samples using Openlab software.
These results match experimental data reasonably well.
Model results match experimental kL data within ±30%.
Experimental samples were run in duplicate.
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