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The mechanical properties of polyamide-6 (PA-6) electrospun nanofibrous mat samples were tested.
To confirm these mat samples were active, they were examined by phase contrast and epifluorescence microscopy with an Olympus BX60 microscope (Center Valley, PA).
Sediment and microbial mat samples were analyzed using scanning electron microscopy (Phillips Field Emission-SEM, FESEM)) combined with energy dispersive analysis of x-rays (EDAX), back-scatter electron (BSE) detection and electron diffraction (ED).
Microbial mat samples were collected in 2004 using a suction sampling apparatus (Emerson and Moyer, 2002) either within the caldera or near the summit of Loihi Seamount from hydrothermal vent sites ranging in depth from 1150 to 1325 mbsl.
Iron mat samples were sheared by vortexing between 10 and 60 seconds, stained with 5 µM SYTO-9 nucleic acid stain (Invitrogen) and passed through a 70 µm mesh-size cell strainer (BD).
Mat samples were studied using light and fluorescence microscopy (Axioskop A1; Axioimager Z1, Carl Zeiss, Germany) in the Microscopy Centre (IC&G SB RAS, Novosibirsk).
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These results suggest that all the fiber mat samples are antimicrobials.
Using a MoFlo™(Beckman Coulter) flow cytometer equipped with a 70 µm nozzle orifice and using a 488 nm laser excitation, fluorescence activated cell sorting (FACS) from LD iron mat samples was done to identify a sort region that would yield ensheathed L. ochracea cells.
In case of mats, samples were extracted by thermomixing at 60°C for 30 min.
The clone libraries from sub-mat samples were dominated by phylotypes of the Eel-2 group, a sister group of the Desulfobulbaceae within the Deltaproteobacteria (Fig. 3a).
The different types of autofluorescence bacterial cells in the microbial mats samples were classified as different genera of cyanobacteria based on microscopic morphology (Table 1).
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