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At Georgetown in 1909, he was called the first quarterback in the East to master the forward pass, several years before Gus Dorais of Notre Dame did so in 1913.
Master the forward pass.
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The β- actin gene was used as a reference (housekeeping) gene (forward primer, ActbF: 5′-TGAAGTGTGACGTTGACATCC-3′ and reverse primer, ActbR: 5′- GCCAGAGCAGTAATCTCCTTC-3′). Reaction mixtures (20 μL) consisted of SYBR® Green PCR Master Mix (Applied Biosystems), the forward and reverse primers and an aliquot of the reverse-transcribed samples (cDNA, 2 μL).
But before you can ski, you have to master the basics: sliding forward on one ski, sliding forward on the other, holding your poles, taking baby steps sideways up hills, and learning the snowplough, always the snowplough.
These were mapped to the direction of travel (i.e. moving the master controller forward moved the tram forward).
Each reaction was carried out in 20 μl of reaction mixture that contained SYBR® Green master mixture, each of the forward and reverse primers at a final concentration of 300 nM, and 5 ng of purified DNA sample.
By using the previously synthesized 2 μl RT stem-looped cDNA products, the quantitative PCR reactions were performed in a reaction mix containing 10 μl of the 2X Master mix, 1 μl of the forward (10 pmol) and 1 μl of the reverse (10 pmol) primers, 0.3 μl (30 nM) of reference dye and 7.7 μl of nuclease-free water.
Before you attempt a handstand forward roll, be sure you've mastered both the handstand and the forward roll separately.
The master equation (Kolmogorov forward equation) takes the form of a Schrödinger equation in imaginary time.
The "AVB" and "PVC" neurons are representatives of the "master controllers" for forward locomotion [32].
Engineering reports commissioned as part of the master plan projected forward to 2035.
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CEO of Professional Science Editing for Scientists @ prosciediting.com