Two polymer concentrations were tested, 20 ppm and 50 ppm, made from a 1000 ppm master solution.
In a parallel, fully independent study using the same master solution, Korschinek et al. [35] have determined a half-life of (1.388 ± 0.018) My.
Employing this factor, an absolute 10Be/9Be ratio of 1.464 ± 0.014 was determined for a first dilution of the 10Be-rich master solution.
Thereafter, the master solution of the polymer which was prepared at 2000 ppm water was injected at controlled flow rates to provide 40 ppm of the polymer in the water phase and the measurement was repeated.
A series of 10 mL portions of master solution of gabapentin with NIN was made up comprising different complementary proportions (0 10, 1 9, 2 8??9:1) in 10-mL calibrated flasks.
At each experimental run, the polymer master solution of 2000 ppm concentration was injected into the flow at a controlled flow rate such that 40 ppm concentration of the polymer was achieved in the water phase.
Series of 10-ml portions of the master solutions of CIN and NQS were made up comprising different complementary proportions (0 10, 1 9,..., 9 1, 10 0, inclusive) in 10-ml calibrated flasks containing 0.5 ml of tris buffer solution (pH 8.5).
Series of 200 μl portions of the master solutions of OLM with pCA were made up comprising different complementary ratios (0 10, 1 9,........., 9 1, 10 0, inclusive) in each well of the 96-microwell assay plate.
Series of 200 μl portions of the master solutions of ATR-Ca with DDQ were made up comprising different complementary ratios (0 10, 1 9,........., 9 1, 10 0, inclusive) in each well of the 96-microwell assay plate.
Series of 200 μL portions of the master solutions of CLM with DDQ were made up comprising different complementary ratios (0 10, 1 9, ……, 9 1, 10 0, inclusive) in each well of the assay plate.
Series of 10-mL portions of the master solutions of LND and FLC were made up comprising different complementary proportions (0 10, 1 9,..., 9 1, 10 0, inclusive) in 10-mL calibrated flasks and the reactions were allowed to proceed for 10 min. The solutions were further manipulated and the fluorescence signals were measured as described under the general recommended procedure.
