In each case the expression of the DosR regulon gene was markedly lower in the DosR mutant, confirming the disregulation of the regulon in the mutant strain.
In this way, master regulons involved in multiple traits (i.e., pleiotropic genes) can be identified, and the amount of epistasis from interacting loci can be uncovered [ 39].
We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability.
The identified master regulons ranked hierarchically highest among all genotyped loci, since they are likely responsible for massive gene expression variation in the pathway and were linked to changes in the studied phenotypes.
These activators control the expression of marA/soxS/rob regulon genes.
acrAB and tolC are marA/soxS/rob regulon genes (Barbosa and Levy 2000).
In addition, it suggested that the mar regulon genes other than acrAB and tolC are barely involved in organic solvent tolerance in JA300 acrR IS marR.
In this study, we did not observe differential expression of any SOS regulon genes.
The adjusted DAF-16 core regulon (genes found in all three species) contains 145 genes (Figure 6a).
Genomic regions with clustered expression and phenotypic trait variation might contain master regulons ([ 46, 79]).
