The service creates a master profile where you can catalog your biography, photos, links to other services, blogs, and even create new kinds of information pages.
The NAD2 test-profile HMM was then searched against the NAD2 "master" profile HMM and the NAD5 profile HMM (generated using all NAD5 sequences) using HHsearch.
A test-profile HMM was generated using one of the subsets, while the remaining nine subsets were used for generating a "master" profile HMM.
Figure 5 shows section 2F from the mouse NotI-EcoRV-HinfI profile with spots 63, 64, and 79 previously identified and the indicated spot not present in the master profile or in a mouse liver profile.
In Fig 2A and 2B, the position of spot 5 is below spots 3 and 6 in the virtual profiles, but in the master profile spot 5 is above spots 3 and 6.
The third panel of Fig 5B shows the composite image of the vRLGS profile with the extra spots retained aligned with the master profile and the candidate virtual spot for the intestine specific spot of interest indicated.
As a further test of the utility of the vRLGS system in the mouse genome, we used vRLGS to identify the sequence of 126 spots from the mouse NotI-EcoRV-HinfI master profile.
We predicted the sequence of spots 36, 56, (not shown in this window) 62, and 68 from section 3D of the master profile based on the virtual spots indicated (Fig 4B).
Spots labeled 1 through 7 are previously cloned RLGS spots allowing the correct identification of each of the corresponding virtual spots, as shown in Fig 2. The swirl shape seen in the master profile is much more evident in Fig 2C and 2D.
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