Sentence examples for master plate for from inspiring English sources

Aliquots were taken from this master plate for each of the analyses.

After 10 minutes, mutants displaying residual activity were identified by formation of a purple color due to the azo dye formed between 1-naphthol and Fast Blue B. The corresponding colony was then isolated from the master plate for validation and further characterization.

We prepared four master plates for each target gene: three plates contained unique non-overlapping siRNA duplexes and the fourth plate contained a pool of the three individual duplexes.

The master plates for the library were copied twice into working copies of the library.

For the construction of the master plates for the side pools, face pools and diagonal pools, for example, all plates which were present in the same position in six consecutive layers of the cube were combined.

Briefly, for testing of the cohort, a master plate of the serum samples was first constructed in a deep well master plate by diluting serum 1/10 in buffer A (50 mM Tris, pH 7.5, 100 mM NaCl, 5 mM MgCl2, 1% Triton X-100 and 0.001% bromophenol red).

Properly exchanged clones were expanded from the master plate and stocked for future use.

Plates were reinoculated to another microtitre plate containing YEPD medium as a master plate and grown for 3 d at 28°.

Colonies were picked from the master plate, and used as DNA template for PCR.

Since growth on a master plate does not give any selection criteria for "transformability", there is always a risk of selecting for yeast cells that are more difficult to make competent for transformation.

At least 14 individual colonies for each construct were plated onto -Ura master plate which was replica plated onto YPD, YPG, and –Ade plates to monitor color pigmentation, petite phenotype and growth on -Ade, respectively.