Sentence examples for master plate and from inspiring English sources

Resistant colonies were grown on a master plate and then replica-plated onto selection plates to determine their ability to induce three independent reporters (HIS3, URA3, and lacZ).

Colonies were picked from the master plate, and used as DNA template for PCR.

Properly exchanged clones were expanded from the master plate and stocked for future use.

Plates were reinoculated to another microtitre plate containing YEPD medium as a master plate and grown for 3 d at 28°.

After 2-3 hours they can be replica plated onto plates lacking the appropriate amino acids and the titrated amount of AT (master plate), and at the same time onto plates covered with a filter membrane.

New 96-well plates were inoculated from the master plates and incubated at 37°C and 600 rpm for 24 h.

For mtDNA studies, 56 colonies (approximately 25 generations from a single cell) of Δ ccm1 non-complemented nascent meiotic segregants from 2nI or 2nB were harvested from YEP with galactose master plates and pooled.

Transformed E. coli TOP10F' cells were plated out on selective LB (Luria-Bertani) medium containing X-Gal, IPTG and either ampicillin (100 μg/ml) or kanamycin (50 μg/ml) [ 38]. White colonies were streaked onto master plates and two sets of nitrocellulose filters (Millipore, Billerica, MA) on LB/kanamycin plates.

After 10 minutes, mutants displaying residual activity were identified by formation of a purple color due to the azo dye formed between 1-naphthol and Fast Blue B. The corresponding colony was then isolated from the master plate for validation and further characterization.

DNA was plated into a 96-well master plate at 5 ng/μL and stored at -20°C until required.

For LIPS analysis, 40 μl of buffer A, 10 μl of diluted sera from the master plate (1 μl equivalent), and 1 × 10 light units (LU) of Renilla luciferase-antigen Cos1 cell extract were added to a final volume of 100 μl) to each well of a standard polypropylene plate.