Sentence examples for master mix in from inspiring English sources

Diluted cDNA samples (1/10) were used for Real time PCR analysis with 200 mM of each gene specific primer mixed with SYBR Green PCR master mix in a final volume of 15 μl following manufacturer's instructions.

cDNA products were mixed with a transcription master mix in the presence of T7 RNA polymerase and Cy3 labeled-CTP and incubated at 40°C for 2 h.

cDNA products were mixed with a transcription master mix in the presence of T7 RNA polymerase and Cy3 labeled-CTP and incubated at 40 °C for 2 h.

Forward and reverse primers (10 μM) and 20 μM labeled probe was mixed with 2× PCR master mix in a total volume of 15 ul and added to 50 ng cDNA in final reaction volume of 25 μl.

QPCR was performed using Power SYBR® green master mix in 25 µl containing 250 ng cDNA, 300 nM forward and reverse primers.

All samples will be reverse transcribed under the same conditions and from the same reverse transcription master mix, in order to minimize differences in reverse transcription efficiency.

Quantitative real-time PCR was performed with specific MGB probes (Taqman; Applied Biosystems and PCRR master mix (Taqman Gene Expression Master Mix), in a commercial thermocycling system (StepOnePlus Real-Time PCR System; Applied Biosystems).

cDNA was amplified using the SYBR Green PCR Master mix in a 7500 Real-Time System (Applied Biosystems, Grand Island, NY, USA).

RT-qPCR was performed using Thermo Fisher 2X SYBR Green Master Mix in a Applied Biosystems 7300 machine.

Real-Time PCR was performed according to the User Manual of RT2 Profiler PCR array system (SABioscience) using SYBR Green PCR Master Mix in a StepOnePlus PCR system (Applied Biosystems).

Real time PCR was performed on diluted cDNA samples using TaqMan Universal PCR Master Mix in ABI PRISM 7500 Real Time PCR System.