Sentence examples for master mix for from inspiring English sources

Primers and probes were pre-mixed with TaqMan Universal Master Mix (for probes) or SYBR®GREEN PCR Master Mix (for primers only) (Applied Biosystems) and applied to 96-well MicroAmp Optical plates (Applied Biosystems).

The master mix for each PCR reaction was composed of Feldan Bio kit (Feldan Bio, Québec, QC, Canada) with 1 unit of taq, 0.5 μM of each primer, 0.2 mM of dNTP, and 1 μl of total AM DNA.

Importantly, ExCyto PCR cells can be added to a master mix for at least 2 hours before initiating thermal cycles (Figure 5).

qRT-PCR was performed in a 10 ul reaction volume in 384 well plates with Power SYBR Green master mix (Applied Biosystems) or Expression Master Mix for TaqMan assays (Applied Biosystems) on the Sequence Detection System 7900 (Applied Biosystems).

We recommend the use of either the Griffiths method or the FastDNA® Spin Kit, in conjunction with an inhibition control, and 2× TaqMan environmental PCR Master mix for extraction of DNA from soil and faeces.

Samples were amplified with Qiagen Master Mix for 35 cycles at 95°C for 30 sec, 62°C for 30 sec and 72°C for 45 sec after an initial denaturation at 95°C for 15 min and followed by a final extension step of 10 min at 72°C.

We diluted the RT product (microarray validation: 25 to 250x, mat2ab: 70x) and used commercial master mixes for quantitative PCR in 5 10 μl reaction volumes (Lightcycler 480 DNA SYBR Green I Master (Roche) or Sybr select Master mix (Life technologies)).

2. SYBR green/primer master mix (a) Prepare SYBR green/primer master mixes for the number of genomic regions analyzed.

Briefly, Multiscribe Reverse Transcriptase (50 U/µL) was added as part of RT master mix, incubated for 25°C for 10 mins, 37°C for 2 hrs, 85°C for 5 mins and stored at 4°C.

The assays were conducted with the following cycling parameters recommended for the master mix: 50 °C for 2 min, 95 °C for 10 min, and 40 cycles of annealing and extension at 95 °C for 15 s and 60 °C for 60 s.

PCR was carried out in 20-μL volumes using Maxima Syber Green PCR Master Mix (Fermentas) for 10 min at 95°C for initial denaturation followed by 40 cycles of 95°C for 15 s and 60°C for 1 min.