Exact(45)
qRT-PCR was performed in a LightCycler 480 instrument (Roche Applied Science, Basel, Switzerland) using a LightCycler 480 Probes Master kit or LightCycler 480 SYBR Green I Master kit (Roche Applied Science), following the manufacturer's protocol.
The master kit, which includes the tracker, charger, clip assembly, and 30 days of service will run you $99.95, with each additional month running you $7.95.
Duplicate samples of the cDNA underwent employing primers and TaqMan probes designed by TIB MOLBIOL and the LightCycler 480 Probes Master Kit (Roche) on a LightCycler 480 System (Roche).
Real-time PCR was performed using Lightcycler 2.0 (Roche) with the SYBR green Taqman master kit (Roche) per manufacturer's instructions.
Aspergillus loads were determined by real time PCR using the LC480 instrument and the probes master kit (Roche applied Science).
Quantitative Real-time PCR was performed with LightCycler® 480 II thermocycler (Roche) using the SYBR Green I Master kit (Roche).
Similar(14)
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Real-time PCR cDNA gene expression was detected using the SYBR Green Master kit and a spectrofluorometric thermal cycler (Applied Biosystems).
Quantitative PCR was performed using SYBR Green PCR Master kit (Applied BioSystems, Foster City, CA).
The PCR was performed in a Lightcycler 1.5 Real-Time PCR System (Roche) using LC Taqman Master kit (Roche).
The cDNA served as a template for real-time PCR using Fast SYBR Green Master kit (Life Technologies).
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